Tissue culture help

What are you soaking in?

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Change the amount or cytokynins or auxins levels in your muskagi agar.

Its the wash cycle. Freshly washed ready to cut.

Where’d you learn the whole process?

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Heres my link.

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:raised_hands:t3::raised_hands:t3: Thanks man

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Man i almost linked this thread shit.


Here’s a tiny cause and effect sheet of the two hormones and how the levels affect the propagation your trying to achieve.

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Flow hoods are so expensive though :sob:

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Ive been playing with trying my own agar mixes/recipes.

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Nahh now small ones are like $500

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What’s that book called?

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Expenses are not a worry. It will be a professional laboratory. I just don’t even know where to start lol. A lot of research to do.

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Thats not a book its part of the tissue culture kits literature. Explaining how it works even comes with 2 kinds of premade agars. One for shoot proliferation and one for rooting only.

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This is helpful.

this lady explains well. Theres is so many videos.

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Wow that’s a lot cheaper than I remember.

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cooperm, aseptic technique is a DISCIPLINE! preybird said it best…sterile, sterile, sterile. Usually developed in a bacteriology or microbiology class. I guess a second best is just search “developing aseptic technique”. Practice with just a few samples originally until you get it down. (In other words don’t start with incubating 200 samples!) good luck!

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@devjyarn actually pulled it off with no failure, his kit was super cheap and simple to use, don’t use bleach use peroxide. So simple anyone can do it I’m told ,even the daft will have no issue unless they can’t read. He also had little to no equipment

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I need the starting points and all the in-between lol. I want to start into gene editing.

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I did my aseptic research this morn. Canna doctor. Thats my new personification lol.

Now to overcome the huskies fur… lol

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