Let's Talk Cannabis Tissue Culture

Join Cannabis Tissue Culture Expert Bill Graham and the Plant Cell Technology Team For This 3-Day Hands-On Master Class


Bill brings 30+ years of experience in the industry and has taught tissue culture master classes worldwide. He even authored the micropropagation section of Ed Rosenthal’s “Cannabis Grower’s Handbook!”


It’s been four decades since the commercialization of tissue culture technology. However, do you know it’s just recently when the technique was introduced in the Cannabis business space?

(By the way, we are only two weeks away from our next Tissue Culture Master Class: Cannabis. Don’t miss out on a seat; book yours today using this link: Tissue Culture Master Class: Cannabis, Washington, Fri Dec 16th 2022, 9:00 am - Sun Dec 18th 2022, 4:00 pm EST | Humanitix :loudspeaker:)

:no_entry_sign: It’s mainly due to restrictions posed on the Cannabis plants.

:white_check_mark: But, increasing legalization of medical Cannabis across the states (so far, around 30 states) has opened the door to enhanced opportunities for businesses in the area.

For many of you who aren’t aware of what tissue culture is, it’s a process in which a few plant tissues are grown in a controlled environment under aseptic conditions. :seedling::petri_dish::test_tube:

For growers looking to increase their product yield and quality, :chart_with_upwards_trend: the technique provides an efficient approach to achieving their goals.

Our latest article talks about how tissue culture can benefit cannabis growers, the most exploited tissue culture techniques, and whether it is a sustainable option.

Click here to learn more on today’s article: Tissue Culture Advantages For Cannabis Growers - Plant Cell Technology | Your partner in plant tissue culture

Let us know in the comments how applying tissue culture to your business model helped grow your Cannabis business.


:seedling: The most common practice in the Cannabis business is growing plants using seeds or cuttings. These practices require growers to maintain all stages of plants ready for times exactly when you need them.

It’s a huge labor involved and a risky condition for the clones to get infected with microbes that spread from plant to plant.

:microscope: For example, suppose you used a mother plant infected with HpLVD, which is identified at a very late stage or often remains unidentified. In that case, all clones generated through the plant will harbor the viroid infection.

:test_tube: These challenges can be solved by using the tissue culture technique.

(If you want to introduce tissue culture in your Cannabis business, enroll in our 3-days comprehensive Cannabis Tissue Culture Master Class starting on December 16th, 2022. Book your tickets using this link: Master Classes)

:question: Many bigger Cannabis businesses have incorporated/incorporated this advanced approach into their business model. But, can hobbyists or small-scale growers possibly bring this technique into their in-house cannabis business?

:page_with_curl: Find the answer in our latest article: Is an In-House Tissue Culture Lab Right for Your Cannabis Business? - Plant Cell Technology | Your partner in plant tissue culture

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It’s the latest thing in cannabis indrustry - at least it has nmade it’s way to hoe cultivators - can purchase tissue cluture "stuff’ now on conputer, might be the "cleanest way to save orginal genetics . have see some home tissue cluture kits on line


kind of like to give it a try and understand that a very clean place to do this !!


Thanks for the information - but the price of $220.00 is a bit high for the average cultivator whom isn’t doing commerical growing but wanting to preserve orginal genetics


Looking to take your cannabis cultivation to the next level? Want to learn about a game-changing technique for maximizing your yield and potency? Look no further than tissue culture! In our latest blog post, we explore the incredible benefits of this cutting-edge method and how it can help you grow healthier, more productive plants.

From faster propagation to enhanced disease resistance, tissue culture offers a range of advantages that every cannabis enthusiast should know about. So if you’re looking to unlock the full potential of your crop, be sure to check out our article and discover the power of tissue culture for yourself: Tissue Culture: An Effective Way to Maximize Cannabis Yield - Plant Cell Technology | Your partner in plant tissue culture


one can order homes tissue culture kit’s -but that’s just the start of it - keeping things clean is the hard part


Can tissue culture help you produce “Elite” Cannabis varieties?

They sure can! And, not only that, but they also help you to preserve your precious strains for a longer time period without the fear of their genetic alterations.

Tissue culture is an advanced approach to plant propagation. And, in the Cannabis industry, not only it can help you produce genetically identical plants at a larger scale but also help you control their terpene profile or produce plants of your desired traits.

How? And, what are the advantages of doing so? Find it in our recent article “How Tissue Culture Can Help Producing “Superior” Cannabis Varieties? - Plant Cell Technology I Your Partner in Plant Tissue Culture”

Further, learn more about the other techniques that can be combined with tissue culture to produce “superior” Cannabis varieties and how they have the potential to revolutionize the field!


If you follow aseptic techniques and the right protocols, this should be a fairly easy upkeep!


:question: Why is Tissue Culture Not Widely Accepted in the Cannabis Industry Despite Its Many Advantages?

Though tissue culture is being studied for decades, however, the novelty of the technique in the Cannabis space is the major limitation of its application in Cannabis production.

The controversy around the plant and legal issues have halted all kinds of research in the Cannabis space. Thus, this delay.

:seedling: However, increasing market demand and legalization in many regions have turned the heads of some big growers towards the implementation of the technology in their business setup.

:page_with_curl: Our recent article talks about the difference between conventional and tissue culture techniques for cannabis propagation, and, if it’s a good idea for your business to install the technology in your setup.

Further, learn from some example companies how they have been utilizing tissue culture for their Cannabis business and if any profit they gained.

:pushpin: Here’s the link: Is Tissue Culture Right for Your Cannabis Business? Here’s What You Need to Know I Plant Cell Technology


Happy 4/20!

:pushpin: Celebrate the day by learning about the science behind the Cannabis plant with our latest blog post: The Science of Cannabis: Understanding the Plant Behind the 4/20 Holiday

:seedling: Cannabis contains a variety of compounds, including cannabinoids and terpenes, that interact with the human body to produce a range of effects.

These compounds are the subject of ongoing research to understand their potential medical benefits.

:potted_plant: Cannabis cultivation techniques have evolved to meet the increasing demand for high-quality, disease-free plants.

From hydroponics to aeroponics to greenhouse cultivation, producers have a range of options for optimizing plant growth and yield.

Disease-free stock production is also important to ensure consistent quality and regulatory compliance.

:pushpin: Check out our blog for more on the science of cannabis and the techniques used to cultivate this fascinating plant.

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:question:What kind of pathogens majorly infect Cannabis?

Cannabis is a plant susceptible to a range of diseases. Some most common pathogens that infect the plants include:

:point_right:t2: Viruses and viroids (such as HpLVD and Hemp Streak virus)

:point_right:t2: Pythium and Fusarium (cause root and crown rot)

:point_right:t2: Botrytis infection (causes grey mold)

The infection causes great loss to the Cannabis propagation businesses. They especially infect plants during their production cycle.

:seedling: One way to control these diseases is the early detection and implementation of pathogen control strategies early during the production cycle (that is within 2-4 weeks).

:pushpin: In our article “Cannabis Propagation: Infection and Prevention”, we dive deeper into what kind of pathogens can infect Cannabis and how these diseases can be controlled and managed: Cannabis Propagation: Infection and Prevention


:male_sign::female_sign: Gender screening is the process of determining the gender of a cannabis plant.

:seedling: It involves identifying the plant’s reproductive organs and determining whether it is male or female.

The process of gender screening involves analyzing the plants’ genetic makeup, physical appearance, and behavior.

:test_tube: Some of the commonly used methods include visual inspection of pre-flowers, PCR (Polymerase Chain Reaction) testing, and chromosome analysis.

:potted_plant: Gender screening is crucial in cannabis cultivation as it helps growers eliminate male plants before they can fertilize female plants, resulting in lower yields and less potent flowers.

It also helps growers maintain a consistent crop by ensuring that they only grow the desired female plants.

:question::question: Interested in learning more about how gender screening can benefit your cannabis business and dive deeper into its methods?

:pushpin: Get everything in our latest blog: Why Is Gender Screening Your Cannabis Plants Important?

Don’t leave the success of your cannabis growth up to chance - make sure you’re properly screening for gender. Check out our blog post for all the information you need to get started.

:point_right:t2: P.S. We are offering a Cannabis tissue culture master class where you can learn about cloning the Cannabis plant using tissue culture to obtain female plants and get hands-on experience screening the plant for gender. Sign up using this link: Master Classes


:microbe: HpLVd viroid is a single-stranded infectious pathogen that causes “dudding” in cannabis plants.

Some apparent symptoms observed in the plant infected by HpLVd include:

:four_leaf_clover: Abnormal branching
:four_leaf_clover: Reduced and stunted trichome production
:four_leaf_clover: Leaf chlorosis
:four_leaf_clover: Stunted growth
:four_leaf_clover: Develop smaller and looser buds
:four_leaf_clover: Brittle stems with less flower mass
:four_leaf_clover: Loss of quality and reduction in yields

The viroid can cause severe yield loss in cannabis-growing facilities worldwide.

Don’t risk losing your crops to this devastating infection.

:pushpin: Check out our latest blog post, “Testing Cannabis Plants for HpLVD and Its Importance” to learn about the detection methods available to catch the infection early and save your plants.

:point_right:t3: Here’s the link: ​Testing Cannabis Plants for HpLVD and Its Importance


:question: What limits you to employing tissue culture in your cannabis cultivation business?

:seedling: Tissue culture techniques have already made an impact on cannabis cultivation by enabling genetic preservation, clean stock production, and scalable propagation.

As the industry continues to evolve, the future potential of tissue culture in cannabis cultivation is vast.

:dna: From genetic enhancement and customization to rapid propagation of rare strains and pharmaceutical production, tissue culture holds the key to advancing the quality, diversity, and sustainability of cannabis cultivation.

However, despite its numerous benefits, tissue culture is not yet being fully utilized in the cannabis business.

:pushpin: In our recent article, we overview the befits, challenges, and future potential of the technique: Assessing the Potential of Tissue Culture in Cannabis Cultivation - Plant Cell Technology | Your partner in plant tissue culture.

But, we’d like to learn about your views on why you think tissue culture is not being used to its full potential in the Cannabis industry.


Lovely articles brother and nice reads.
It is also worth noting that many cannabis farmers are being forced to grow their crops indoors due to the effects of climate change hoax. This means that they must rely on artificial lights and environmental controls, which can be costly and difficult to manage. In this scenario, tissue culture techniques can be particularly beneficial, as they allow for precise control over the growing environment and the ability to produce large numbers of identical plants.

in the past, tissue culture techniques were commonly used when working with regular (non-feminized) cannabis seeds, as they allowed for the identification and removal of male plants, which can lower the quality and potency of the final product.

But what I’ve found with researching is that the development of new strains that are feminized and “cannot be pollinated” (seedless cannabis) tissue culture techniques may become less important in this regard. This would be extremely good and beneficial for Canadian farmers but the timming is off. Nevertheless, the benefits of tissue culture, such as faster and more efficient plant propagation, as well as the ability to produce disease-free plants, will still make it a valuable tool in the cannabis industry.

While the lack of funding and support for cannabis tissue culture techniques may be a current challenge for the industry, this is likely to change in the near future as the benefits of the technology become more widely recognized and understood. With the increasing legalization and demand for high-quality, consistent cannabis products, tissue culture will play an essential role in driving the growth and development of the industry.

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:seedling: Discover the latest breakthroughs and scientific advancements in the field of cannabis research. :bulb:

:test_tube: From understanding the therapeutic potential of cannabinoids to unlocking the secrets of cannabis genetics, scientists are making remarkable strides in unraveling the mysteries of this versatile plant.

With evolving legal frameworks and groundbreaking clinical research, the realm of cannabis investigation has flourished.

:microscope: In a short span of time, our comprehension of the plant’s compounds has transformed from rudimentary to revealing pivotal links between cannabinoids and human physiology.

This expanding knowledge is not only unraveling the mysteries of cannabis but also fueling the growth of STEM job opportunities.

:page_facing_up: Our latest blog post delves into the incredible strides made in cannabis research, uncovering key discoveries that have the potential to revolutionize medicine, agriculture, and more. :seedling:

:pushpin: Click the link to read the full blog post: ​3 Significant Scientific Advances in Cannabis Research

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