Anyone have experience with tissue culture?
I’m going to start experimenting with it. Anyone have the process of it down? Any tips or tricks and help would be great
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Yes i have experience so does @charlieseeds. Do you have laminar flow hood? This tissue culture needs to be sterile sterile sterile. Anyone on your staff practice aseptic techniques? Are you just multiplying shoots and leaves to make more shoots to clone or are you just doing tissue culture to rooting clones.
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Laminar flow hood?
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We all try to stay super clean and sterile in the clone room. But they want to build me a new lab with a shower and only I will have access. I have little to no knowledge in the tissue culture process
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Shoots to clones
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How would you do tissue culture to rooting clones?
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What are you soaking in?
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Change the amount or cytokynins or auxins levels in your muskagi agar.
Its the wash cycle. Freshly washed ready to cut.
Where’d you learn the whole process?
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Heres my link.
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Thanks man
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Man i almost linked this thread shit.
Here’s a tiny cause and effect sheet of the two hormones and how the levels affect the propagation your trying to achieve.
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Flow hoods are so expensive though 
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Ive been playing with trying my own agar mixes/recipes.
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Nahh now small ones are like $500
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What’s that book called?
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Expenses are not a worry. It will be a professional laboratory. I just don’t even know where to start lol. A lot of research to do.
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