So you need a pressure cooker unless you have a autoclave. This is needed to steralize the media the vessels and paper towels and to make steralized water.
Next step is media preparation.
Media prep is best done in a clean kitchen. Media is mixed, distributed into vessels and sterilized by autoclave, pressure cooker and or microwave **micro wave is the last choice. Not very good but works in a pinch.
Extra water, paper towels, instruments, tools are steralized before transfer into the laminar flow bench or hood. Give yourself enough room to mix and move around freely.
You need MS or media salt hormone and a PPM preservative mix, and Gel-agar powder, ph control kit and ph test paper or ph meter. Vessels or tubes or jars that are autoclavable .
To make the media gel
2. 1 quart or 1liter ot 1000ml pyrex beaker.
3. Magnetic stirring plate
4. Distilled water.
5. 2 tablespoons white sugar.
6. Ms media salts-ppm preservative liquid
7. Agar gel
Take the beaker and put on the magnetic stirrer plate turn it on. Add 900ml of distilled water. Then add the 2 tablespoons of sugar. Let the sugar disolve then add the ms-hormone-ppm liquids. The most common establishment and multiplication media is called B1 and is made from 1mg BA, 0.1 mg NAA and 1ml PPM per liter. This is a standard mult formula and is the most common starting media.
Then make sure the water level after adding solution is now at 1000ml if not add more water. Now ph this to 6.0 using the ph control kit. because adding the gel agar will decrease the PH a few 0.1 of a point. Now turn the heating plate on if your magnetic stirring plate has that option. Now get your rack of liquid tubes-jars ready if so make sure you PH the water before adding the agar.
Agar is special because it must be added to cold water warm water makes it clump up and doesnt dissolve in hot water. When added to the solution the agar will fall to the bottom and separate from the solution. And must be stirred while adding to the vessels. This is why a magnetic stirring plate is so awesome and the heat makes the agar mix with the solution more uniformly.