I like how you presented your experience here…I am hoping you get your lesson…lol…I am waiting to see this through to the end…before I even decide if I want to try it…lol…
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It can get fairly expensive by the time you get your chemicals. Tissue culture takes quite a bit of work after you start your multiplying phase you have to keep changing out the explants about every 6 to 8 weeks and cutting off the new growth from the explants and putting the new growth into your test tubes , pretty soon you have hundreds of test tubes then they have to have a gel change so pretty soon it gets un-manageable. The last thing I tried was to buy a rooting system for oasis they make special cubes for tissue culture rooting the cubes hold 15 explants the material alows more air to the rooting site and you don’t need gel which blocks the air needed to get roots. I have only used the oasis cubes once and it failed I need to get the chemical ratios right. If anybody has any questions I will try to answer from my experience.
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Yeah I was letting @preybird complete his round and watch it here… but I think you made my mind up and so did @preybird with his silver thiosulphate conversation on my one thread…
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Very interesting. I am in the middle of this. It does get very very expensive. I can get the chemicals for around a hundred dollars. But whats annoying is just like you said gel swaps are no fun. Also the gel can only feed for about 2.5 weeks. Then your leafs will go white opake and then your too late. Im trying to practice the phase where i go from multiplication to rooting. And actually i will be do a rooting gel varience using cloxed gel and the other chems i have. To test whats working. I had great succes in multiplication. But the next step is tricky. Because the tubes are not sterile anymore and you have to steralize everything again. I was talking to @Ladithief about just going the femized route and making the female pollinate herself and get the seeds using Silver thiosulfate solution to block the femal hormone. And force it to make femanized pollen male flowers. Otherwise its clones. This is why i also permaclone. I never keep a mother. Every new clone is a mother. Its easier to rotate stock perse than keep a big mother. If it gets bugs you could lose it. Completely.
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There is also a chemical and tissue culture supply company( phytotechlab.com) they also carry pre made test tubes that are sterile and has the chemicals in gel, they are a bit pricey but easy to use. search their website for cannabis they also carry a Hemp kit.
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@preybird I think sent me that site for Silver Thiosulphate for femming…
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Yes i did send that link they have the best costs for product. And they will sell you anything!
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Hey @charlieseeds thankyou for your input here it is greatly appreciated. I have never done this but i love to learn cool new things and growing knowledge is what im having.
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Nice tek write up.
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Thankyou!
Well i went on a weird tangent and started testing how long a cutting can live in multiplication gel. I dont know why i did. But now im am realizing that i was intending to do longer term storage of a mother plant in the gel. And a light bulb came on in my brain about how often am i going to have to make this gel and how long can a cutting go if say you had to leave it. Well im seeing about 3 weeks with 20ml of gel. Then the gel shrinks and starts to get used up. If you let it go further you will get mold. Because the plant tissue will turn white and opaque as the gel loses sugar potentcy. I also noticed the tube that had more gel was lasting longer. And then i though man i need to do more testing. Because my cuts made 3 shoots each. And things grew a lot faster than i thought they were going to. And i think i had my temps way off. I found out my main bloom tents fan was so powerfull that the clone tents fan that goes to the same exit duct. And it was being blown back past the booster fan and back through the filter and into tje clone tent raising the temp a lot. I could not figure out what was happening. Then i took the filter off when it was running and found the issue. Needless to say i was not to happy and will be practicing this more. I need to talk to a couple people i know have knoweledge here. Im hoping for a recipe someone has used thats better than this current one. I have 2 recipes for agar. 1 rooting and 1 multiplication. I would like one that has a little of both. Shoots and roots? I wish i had more time to research and test more mixtures. But i simply do not have the time. And it’s my busy season at work and my race bikes need work before i can go to the track. And i neeeeed to go on vacation. And i got shit to do damn it.
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Keep us posted… I seen Sasquatch clones take 4000 clone this way from SoLo farms in Oregon… they do it for many farms… I wonder how often they have to change it… that would be a nightmare with 100,000 or more… unless they know something that others don’t… nice thread @PreyBird1 one of my favourite…
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This is a hemp how to but it works for Cannabis
https://phytotechlab.com/mwdownloads/download/link/id/2343/
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Well i have a lab kit and have been practicing. Have you tried any different hormone mixes. A little more of this a little less of that? What recipes did you use when you were still doing it. @charlieseeds i appreciate your links and experience. Any tips? Anything you would do differently?
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I tried many too many the basal salt with the Meta Topolin keeps the callas that forms during multiplying from getting to big. I was unable to get roots every step besides the last went well. I need to work on that I will after this next crop finishes. Being a breeder takes all my time and so does developing new strains.
I’m shooting for next year to go public with my strains.
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Nice well im on it man. Im trying some mixes. Ill update as i can. Im going to do a rooting agar.
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Holy cow! That is amazing and just blew me away. One heck of a set up for home! Super impressive! Also very god run down and techniques for tissue culture!! Great job man! Keep us all up date.
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Wow thanks for the compliment. Just an amazing plant. And this place is amazing to get all of us like minded people together.
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Hey @PreyBird1 thank you very much for doing this tutorial, it’s been really helpful to me for getting to understand such a cool alternative to cloning! I am fairly new & have a quick question, I see that your plants are have calloused & was wondering if your mix only had the “MS media” & all the other ingredients you’ve listed above or was there a different hormone you used in order to induce this formation? Thank you in advance for your help!
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Yes i used a branching and shoot muskagi agar for multiplication. Not a rooting agar mix that comes after in the next stage.
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