None of these had leaves when i started.
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This is all so very interesting and awesome AF
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Its is very fun to play scientist! Im enjoying it immensely. I want to take a picture of all my plants in small jars and then put them into a huge empty 8x8 tent to show how small an area they take up. So small and soooo cool like micro machines when we were kids.
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Micro machines what are those? Those tubes are filling up fast. Very intriguing.
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Outstanding job, @PreyBird1. Well done. Very impressive.
Do you have prior laboratory experience? It definitely looks like you are using good laboratory practices. 
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I do not have lab experience but. I reasearched it for months and months and spend countless hours learning and trying to get better at aseptic techniques. I watched probably 50 videos for hour and hours and im not kidding. I like to do things as well as i can. Because if i do it wrong ill scrap it and start over. So i make sure i do it the way it should be done and using the correct tools equipment. It garuntees better success.
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Very nice @PreyBird1… some day if I have to great way to keep a mom you don’t need for a long time if I heard right…lol… Sasquatch clones does up in Oregon by the masses for many farmers…
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Sasquatch? I just got thugpug official seeds
Today in an auction.
So we
Good lol
Packee
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You can have a mother for a longggg time this way. At little cost. This is how i will store my living strains. I already have 15 female plants and 1 male. And i now have 3 more males that will go into my library. And the aficionados white caviar f3 male and female are going to be put into tissue culture and im going to mate the pair of them and make more seeds. But the whole reason i went with the white caviar was so i could cross it with the white runtz. And now i may cross it with the regular Runtz.
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That will be a great cross bro 
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Looks great… you are going to do well. Thanks for posting!
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So the tissue cultures were done with a BA hormone wich is the branching and shoots hormone. You can also make your multz gel in any combination you would like. Next i will be making the rooting gel multz using a auxin hromone.
Here is a cytokinin chart for what the hormones can do.
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Excellent work @PreyBird1!
That will be my lab next year!
(I worked with orchids and bamboo with this technique).
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Wow its very similar. And thankyou @paulcoyote
I find it interesting and in depth.
Depending what type of growth you want. Whether is shoots and tips or if you want roots. Here is a chart of what cytokinins and auxins will produce depending on your mixtures of these hormones. Its funny because a lot of us growers have the main ingredients for making multz gel. Rooting hormone like clonex and most of the nutrients and basalts needed are already in a lot of the all of our garden supplies. We all use nutrients. You just need to experiment with certain levels of these hormones. For instance you can take a tip culture and after it has made shoots put it into a rooting multz to make it grow roots then plant it in a media. But if you just want to have more then just use a branching and tip hormone dominate gel mixture and youll get lots of shoots and meristems to harvest. Make 1 jar let it get a bunch of shoots then take that plant clump out and divide into more jars. When ready to root and transfer to grow room/greenhouse just use a different mix to make it root fast. Very fun to dabbel with this technology and techniques.
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Id like to get smaller and maybe get in gene fusion and recombinant DNA mixing or protoplast. Im going to keep trying things and growing my skills. And learning more and more. Once i have multiplied the tubes i have now. I am going to take a slice of just the callus base of the shoot and try snd culture it into leaves and meristem and all the way up to a full size plant. I will be documenting all my progress and photo’s of my work here for everyone to enjoy and maybe get inspired to try something wayyy beyond the normal aspects of plant propogation that a regular grower would never consider and maybe never even knew existed! I didnt and i was sooooo fascinated.
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Yes Sasquatch… and @PreyBird1 that’s what I thought I heard… I also don’t have time to learn it as I do have planned as that’s my winter time habit… educating myself…no time this time of year…lol…
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Ok update guys/gals. Here are the cultures on the 9th.
Now if you’ll notice there are zero roots. Were just multiplying shoots at this point for further dissection to make multiple single tubes. Then those will be put into the rooting gel when ready to move towards being out of the tubes and hardening them to go into a normal enviroment. Since we as growers have a lot of the ingredients for the rooting stage already in there own garden supplies. I wanted to make and easy to make compund using clonex gel as the rooting hormone in the agar gel base so any of us here could make it more cost effectively and maybe a little easier to make instructions. Im waiting on a some supplies right now for making lots more cultures and im going to play around with the cytokinin vs auxin ratios and see what i can come up with. Im looking for a recipe for a balance of rooting and multiplication agar gel. More on this later after i do some more testing when i get my supplies.
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Super! Cannabis industry has a lot to offer!
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I have been working with tissue culture on and off for years. Had great success except for the final stage getting the explants to produce roots . There are very few documents on how to get them to root. Let me know how this step goes for you.
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